exRNAdisease

Entry Detail

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General Information

Database ID: exR0359486 RNA Name: AGO1 RNA Type: mRNA Chromosome: chr1 Starnd: + Coordinate: Start Site(bp): 35869808 End Site(bp): 35930532 External Links: ENSG00000092847

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand MT-ND4L chrMT 10470 10766 + ESPL1 chr12 53268299 53293638 + SAMD4B chr19 39342396 39385710 + CANX chr5 179678628 179731641 + NFATC2IP chr16 28950807 28967097 + LAMTOR1 chr11 72085895 72103297 - PSMD2 chr3 184299198 184309050 + AP1B1 chr22 29327680 29423179 - PJA2 chr5 109334713 109409974 - KDM4B chr19 4969113 5153598 + REC8 chr14 24171853 24180257 + PTMA chr2 231706895 231713541 + HLA-A chr6 29941260 29945884 + FTO chr16 53701692 54158512 + KRIT1 chr7 92198969 92246166 - DNMBP chr10 99875577 100009947 - AP002990.1 chr11 62559603 62591531 - RPL36AL chr14 49618530 49620626 - SMS chrX 21940709 21994837 + SPINT2 chr19 38244035 38292615 + KLHL36 chr16 84648511 84667686 + PANX1 chr11 94128841 94181968 + CALM1 chr14 90396502 90408268 + PRPF40A chr2 152651593 152717997 - AGO2 chr8 140520156 140635633 - NEURL4 chr17 7315628 7329393 - RPL32 chr3 12834485 12841582 - RPL8 chr8 144789765 144792587 - LCOR chr10 96832282 96995959 + SND1 chr7 127652194 128092609 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC023509.1 chr12 53441741 53467528 + AC007347.1 chr16 54033997 54054583 - AC022034.3 chr8 53394110 53484067 - LINC01641 chr1 227393554 227431035 + TUG1 chr22 30969245 30979395 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.