exRNAdisease

Entry Detail

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General Information

Database ID: exR0359487 RNA Name: AGO2 RNA Type: mRNA Chromosome: chr8 Starnd: - Coordinate: Start Site(bp): 140520156 End Site(bp): 140635633 External Links: ENSG00000123908

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand RPL13 chr16 89560657 89566828 + SPECC1 chr17 20009344 20319026 + ENO1 chr1 8861000 8879190 - EIF2AK1 chr7 6022247 6059175 - RAB5B chr12 55973913 55996683 + PLXNB2 chr22 50274979 50307646 - LENG8 chr19 54448887 54462037 + HNRNPU chr1 244840638 244864560 - SMAD7 chr18 48919853 48952052 - BRIX1 chr5 34915677 34925996 + CD3EAP chr19 45406209 45410766 + PSMC3IP chr17 42572315 42577831 - CCDC137 chr17 81666737 81673904 + TSPYL1 chr6 116267760 116279903 - TIAL1 chr10 119571802 119597029 - AGO1 chr1 35869808 35930532 + GID8 chr20 62938147 62948475 + ERBB2 chr17 39687914 39730426 + TSPAN13 chr7 16753755 16784536 + RMDN3 chr15 40735884 40755851 - TFDP1 chr13 113584721 113641473 + PLXNA1 chr3 126988594 127037392 + PDLIM1 chr10 95237572 95291012 - MCC chr5 113022099 113488823 - RANBP9 chr6 13621498 13711835 - NPC1 chr18 23506184 23586506 - ILF3 chr19 10654261 10692417 + APRT chr16 88809339 88811937 - SMARCD2 chr17 63832081 63843065 - TCF4 chr18 55222185 55664787 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC068831.7 chr15 91022766 91023200 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.