exRNAdisease

Entry Detail

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General Information

Database ID: exR0359488 RNA Name: AGO3 RNA Type: mRNA Chromosome: chr1 Starnd: + Coordinate: Start Site(bp): 35930718 End Site(bp): 36072500 External Links: ENSG00000126070

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand DVL3 chr3 184155377 184173614 + FBXL19 chr16 30923055 30948783 + ATAD3B chr1 1471765 1497848 + PPP1R12C chr19 55090914 55117637 - TXNIP chr1 145992435 145996579 - WASF2 chr1 27404230 27490167 - ADORA2B chr17 15944917 15975746 + MGLL chr3 127689062 128052190 - PDIA5 chr3 123067025 123225227 + SSR3 chr3 156540140 156555184 - MYCBP2 chr13 77044657 77327094 - SNRPN chr15 24823637 24978723 + DVL1 chr1 1335276 1349418 - RPL13 chr16 89560657 89566828 + ZNF775 chr7 150368790 150398630 + SLC5A6 chr2 27199587 27212958 - CAPN15 chr16 527712 554636 + PAICS chr4 56435741 56464579 + ALKBH6 chr19 36009120 36014239 - CHD3 chr17 7884796 7912760 + FAM126B chr2 200973718 201071671 - RIMBP3B chr22 21383374 21389478 + AC073111.4 chr7 150400702 150412470 + SPAG5 chr17 28577565 28599025 - YBX1 chr1 42682418 42703805 + PPA2 chr4 105369077 105474067 - MT-ND4L chrMT 10470 10766 + VPS13B chr8 99013266 99877580 + RPS3 chr11 75399515 75422280 + DIP2A chr21 46458891 46570015 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC002116.1 chr19 36008638 36014235 - AC007952.4 chr17 19112000 19112636 - AC108136.1 chr8 122485515 122489815 - AC079949.1 chr12 127142029 127146532 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.