exRNAdisease

Entry Detail

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General Information

Database ID: exR0359489 RNA Name: AGO4 RNA Type: mRNA Chromosome: chr1 Starnd: + Coordinate: Start Site(bp): 35808016 End Site(bp): 35857890 External Links: ENSG00000134698

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ADAM19 chr5 157395534 157575775 - MT1G chr16 56666731 56668065 - POLD2 chr7 44114681 44124358 - UBXN6 chr19 4444999 4457794 - RPLP2 chr11 809965 812880 + TATDN2 chr3 10248023 10281218 + PTGES2 chr9 128120693 128128462 - VAMP3 chr1 7771296 7781432 + CBFA2T2 chr20 33490075 33650036 + TMEM132A chr11 60924463 60937159 + MT-CO3 chrMT 9207 9990 + TRIM73 chr7 75395063 75410996 + SFPQ chr1 35176378 35193145 - PMEL chr12 55954105 55973317 - RPL37 chr5 40825262 40835222 - GATA2 chr3 128479427 128493201 - PTMA chr2 231706895 231713541 + SRGN chr10 69088103 69104805 + YBX1 chr1 42682418 42703805 + SLC38A2 chr12 46358188 46372773 - HNRNPF chr10 43385617 43409166 - ANKH chr5 14704800 14871778 - AGO3 chr1 35930718 36072500 + OSBPL8 chr12 76351797 76559809 - DDX5 chr17 64498254 64508199 - SDF4 chr1 1216908 1232067 - XPO1 chr2 61477849 61538626 - LGMN chr14 92703807 92748679 - ARL8B chr3 5122245 5180912 + GGCT chr7 30496621 30504841 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC124944.3 chr3 195900986 195903417 + NEAT1 chr11 65422774 65445540 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.