exRNAdisease

Entry Detail

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General Information

Database ID: exR0359491 RNA Name: AGPAT3 RNA Type: mRNA Chromosome: chr21 Starnd: + Coordinate: Start Site(bp): 43865223 End Site(bp): 43987592 External Links: ENSG00000160216

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand TUBB chr6 30720352 30725426 + WDR54 chr2 74421678 74425755 + RPLP1 chr15 69452814 69456205 + CARD9 chr9 136361903 136373681 - VAPB chr20 58389229 58451101 + IPO9 chr1 201829149 201884291 + KCTD3 chr1 215567304 215621807 + IMP3 chr15 75639085 75648706 - CLN3 chr16 28474111 28495575 - OSGEP chr14 20446401 20455089 - PPRC1 chr10 102132994 102150333 + CKLF chr16 66552563 66566251 + PDIA4 chr7 149003062 149028662 - RBM15 chr1 110338506 110346681 + RPL27A chr11 8682788 8714759 + ANKRD28 chr3 15667236 15859771 - HLA-B chr6 31269491 31357188 - BHLHE40 chr3 4979437 4985323 + SQSTM1 chr5 179806398 179838078 + RER1 chr1 2391775 2405442 + LAMC1 chr1 183023420 183145592 + RUNDC1 chr17 42980565 42993690 + POMK chr8 43093506 43123434 + GON4L chr1 155749662 155859400 - ACAP3 chr1 1292390 1309609 - DTNBP1 chr6 15522801 15663058 - PIK3R2 chr19 18153163 18170532 + ACIN1 chr14 23058564 23095614 - AC007192.1 chr19 18153158 18178117 + SPC24 chr19 11131520 11155808 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL049840.4 chr14 103694516 103695050 - AC010326.4 chr19 57867038 57868172 - AL355075.4 chr14 20343048 20343685 - LINC00665 chr19 36313067 36331770 - LINC01754 chr20 47391925 47412327 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.