exRNAdisease

Entry Detail

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General Information

Database ID: exR0359494 RNA Name: AGRN RNA Type: mRNA Chromosome: chr1 Starnd: + Coordinate: Start Site(bp): 1020120 End Site(bp): 1056118 External Links: ENSG00000188157

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCA2 chr9 137007234 137028922 - LDB1 chr10 102106489 102120368 - PPP1R14B chr11 64244479 64246943 - SAE1 chr19 47113274 47210636 + BTBD2 chr19 1985438 2034881 - ARFGAP2 chr11 47164299 47177125 - HAX1 chr1 154272589 154275875 + GPR153 chr1 6247353 6261098 - SHMT2 chr12 57229573 57234935 + INPPL1 chr11 72223701 72239147 + TSPAN4 chr11 842812 867116 + ZCCHC14 chr16 87406246 87493024 - CKAP5 chr11 46743048 46846308 - POP7 chr7 100706121 100707486 + PSMC5 chr17 63827152 63832026 + VPS26A chr10 69123512 69174412 + AP002990.1 chr11 62559603 62591531 - PAK6 chr15 40217428 40277487 + ARFGEF1 chr8 67173511 67343781 - TECR chr19 14517085 14565980 + TLN1 chr9 35696948 35732195 - CHD2 chr15 92900189 93027996 + TOE1 chr1 45340052 45343973 + STK11 chr19 1177558 1228431 + FGFRL1 chr4 1009936 1026898 + CYB5R3 chr22 42617840 42649392 - OTUD5 chrX 48922028 48958386 - EAF1 chr3 15427598 15450635 + EFTUD2 chr17 44849948 44899445 - DERA chr12 15911302 16037381 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-let-7a-5p chr9 94175962 94175983 + hsa-miR-184 chr15 79209840 79209861 + hsa-miR-654-5p chr14 101040234 101040255 + hsa-miR-541-3p chr14 101064548 101064569 + hsa-miR-5100 chr10 42997630 42997651 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC002470.2 chr22 20979462 20998121 + AC005332.6 chr17 68126666 68129586 + AC079922.2 chr2 112641832 112645720 - AC114271.1 chr19 10333436 10336248 + AD000090.1 chr19 35557956 35581954 + AL031963.3 chr6 3068045 3068894 - AC079949.1 chr12 127142029 127146532 - AL645608.6 chr1 904834 915976 + AL662884.1 chr6 32184733 32185882 - AP001107.5 chr11 66409158 66417137 + BACH1-IT2 chr21 29370497 29373709 + EPB41L4A-AS1 chr5 112160526 112164818 + MIRLET7BHG chr22 46053869 46113928 + NOP14-AS1 chr4 2934882 2961738 + NORAD chr20 36045618 36051018 - SNHG3 chr1 28505980 28510892 + XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.