exRNAdisease

Entry Detail

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General Information

Database ID: exR0359529 RNA Name: AK2 RNA Type: mRNA Chromosome: chr1 Starnd: - Coordinate: Start Site(bp): 33007940 End Site(bp): 33080996 External Links: ENSG00000004455

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand IFI30 chr19 18173162 18178117 + C19orf53 chr19 13774456 13778773 + RBM10 chrX 47145221 47186813 + RPL5 chr1 92832013 92841924 + SAP30BP chr17 75667251 75708062 + PPP2CA chr5 134194332 134226073 - RPLP1 chr15 69452814 69456205 + REXO1 chr19 1815248 1848483 - ARL2BP chr16 57245259 57253635 + GTF3C5 chr9 133030675 133058503 + GRID1 chr10 85599552 86366795 - OGFOD3 chr17 82389210 82418637 - MRPL4 chr19 10251901 10260055 + EIF4E chr4 98871684 98930637 - DDIT4 chr10 72273924 72276036 + MYBBP1A chr17 4538897 4555631 - MZT1 chr13 72708367 72727629 - AC011448.1 chr19 19516227 19536076 + MTRNR2L1 chr17 22523111 22524663 + CDC42SE1 chr1 151050971 151070325 - HAUS1 chr18 46104378 46128333 + POMP chr13 28659104 28678959 + AP2A1 chr19 49766968 49807113 + HLA-B chr6 31269491 31357188 - OCIAD1 chr4 48805212 48861817 + MAL2 chr8 119165034 119245673 + RPS5 chr19 58386400 58394806 + HLA-DQB1 chr6 32659467 32668383 - STRAP chr12 15882387 15903478 + SLC25A37 chr8 23528956 23575463 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-204-5p chr9 70810031 70810052 - hsa-miR-211-5p chr15 31065095 31065116 - hsa-miR-141-3p chr12 6964155 6964176 + hsa-miR-299-3p chr14 101023832 101023853 + hsa-miR-1224-5p chr3 184241405 184241423 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC009133.5 chr16 29808679 29812227 + AL121985.1 chr1 160670778 160699761 + GUSBP11 chr22 23638487 23717356 - LINC02381 chr12 54126082 54147485 + LINC02539 chr6 137730170 137792835 - MALAT1 chr11 65497688 65506516 + SNHG17 chr20 38419638 38435409 - SNHG5 chr6 85650491 85678932 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.