exRNAdisease

Entry Detail

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General Information

Database ID: exR0359531 RNA Name: AK4 RNA Type: mRNA Chromosome: chr1 Starnd: + Coordinate: Start Site(bp): 65147549 End Site(bp): 65232145 External Links: ENSG00000162433

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand MYH9 chr22 36281277 36388067 - MAPKAPK2 chr1 206684944 206734283 + RER1 chr1 2391775 2405442 + RELA chr11 65653597 65663090 - SFXN1 chr5 175477062 175529742 + SMN2 chr5 70049612 70078522 + TNIP1 chr5 151029945 151093577 - TM9SF3 chr10 96518110 96587452 - DPH2 chr1 43970000 43973369 + STAT1 chr2 190908460 191020960 - CD22 chr19 35319261 35347361 + IGF2 chr11 2129112 2141238 - MTRNR2L1 chr17 22523111 22524663 + UBE3A chr15 25333728 25439051 - NFATC2IP chr16 28950807 28967097 + RPL14 chr3 40457292 40468587 + SDCBP chr8 58552924 58582859 + DHX15 chr4 24517441 24584554 - TFRC chr3 196027183 196082096 - PPP5D1 chr19 46480796 46601200 - YWHAB chr20 44885702 44908532 + ACTG1 chr17 81509971 81523847 - RBCK1 chr20 407498 430966 + TMED5 chr1 93149742 93180516 - CBX3 chr7 26201162 26213607 + APOE chr19 44905791 44909393 + RPL15 chr3 23916545 23924374 + HLA-B chr6 31269491 31357188 - PATZ1 chr22 31325804 31346346 - CD47 chr3 108043091 108091862 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-362-3p chrX 50009005 50009026 + hsa-miR-556-3p chr1 162342600 162342621 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL359924.1 chr1 237862175 237928321 + AL627309.5 chr1 141474 173862 - BX284668.2 chr1 16870945 16883659 + LINC01641 chr1 227393554 227431035 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.