exRNAdisease

Entry Detail

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General Information

Database ID: exR0359539 RNA Name: AKAP13 RNA Type: mRNA Chromosome: chr15 Starnd: + Coordinate: Start Site(bp): 85380571 End Site(bp): 85749358 External Links: ENSG00000170776

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ZFAND6 chr15 80059568 80138393 + FNBP1 chr9 129887187 130043194 - IPO13 chr1 43946950 43968022 + NUDT3 chr6 34279679 34392669 - CXXC4 chr4 104468308 104494901 - RPS4X chrX 72255679 72277248 - OSTF1 chr9 75088514 75147265 + CLEC16A chr16 10944564 11182186 + HPS3 chr3 149129638 149173732 + GCSAM chr3 112120839 112133270 - CAPN1 chr11 65180566 65212006 + RBM3 chrX 48574449 48581162 + GOLGA8B chr15 34525207 34588503 - HAUS5 chr19 35612735 35625355 + CENPA chr2 26764289 26801067 + CTDNEP1 chr17 7243591 7252491 - RBM47 chr4 40423267 40630875 - PTEN chr10 87863625 87971930 + ZNF277 chr7 112206695 112343934 + SLC17A9 chr20 62952707 62969585 + SNTB2 chr16 69187129 69309052 + CGGBP1 chr3 88051944 88149885 - ARIH1 chr15 72474330 72602987 + ST6GALNAC6 chr9 127885321 127905408 - ATP5PF chr21 25716503 25735673 - PSMG1 chr21 39174769 39183488 - PCYT2 chr17 81900965 81911464 - CHD3 chr17 7884796 7912760 + ZNF354B chr5 178859953 178888122 + TMCC2 chr1 205227946 205285632 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-449a chr5 55170586 55170607 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC011603.2 chr12 49090208 49093312 + AC016590.2 chr19 37337236 37337743 + AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + AL157935.2 chr9 127934503 127940952 + AL355075.4 chr14 20343048 20343685 - LINC01783 chr1 16533886 16536172 - LINC02249 chr15 30195809 30217552 + TMEM99 chr17 40819106 40836270 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.