exRNAdisease

Entry Detail

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General Information

Database ID: exR0359541 RNA Name: AKAP17A RNA Type: mRNA Chromosome: chrX Starnd: + Coordinate: Start Site(bp): 1591604 End Site(bp): 1602520 External Links: ENSG00000197976

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand LASP1 chr17 38869859 38921770 + TKT chr3 53224712 53256052 - RPL13 chr16 89560657 89566828 + RAB11FIP3 chr16 425649 523011 + UNCX chr7 1232872 1237326 + ARFRP1 chr20 63698642 63708025 - UBA5 chr3 132654446 132678097 + FDPS chr1 155308748 155320666 + BSG chr19 571277 583493 + CSDE1 chr1 114716913 114758676 - PI4KA chr22 20707691 20859417 - FBXW5 chr9 136940435 136944738 - MAP3K15 chrX 19360056 19515261 - PGM1 chr1 63593411 63660245 + NOA1 chr4 56963350 56977606 - CDC42EP5 chr19 54465026 54473296 - HNRNPA1P48 chr16 51553436 51647132 + TOB1 chr17 50862223 50867978 - TMUB1 chr7 151081085 151083493 - GNAS chr20 58839718 58911192 + EEF2 chr19 3976056 3985463 - TMSB4X chrX 12975110 12977227 + MATR3 chr5 139273752 139331671 + SIGMAR1 chr9 34634722 34637809 - HLA-DMA chr6 32948613 32969094 - ZNF48 chr16 30378106 30400108 + STARD3NL chr7 38178222 38230671 + SNRPN chr15 24823637 24978723 + TJP2 chr9 69121264 69274615 + CLIC4 chr1 24745382 24844321 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC124068.2 chr15 89335053 89336161 + AD000090.1 chr19 35557956 35581954 + AL122017.1 chr6 81527102 81534915 - BX284668.2 chr1 16870945 16883659 + ZNF236-DT chr18 76794732 76822295 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.