exRNAdisease

Entry Detail

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General Information

Database ID: exR0359546 RNA Name: AKAP8 RNA Type: mRNA Chromosome: chr19 Starnd: - Coordinate: Start Site(bp): 15353385 End Site(bp): 15379798 External Links: ENSG00000105127

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ANAPC7 chr12 110372900 110403730 - SHQ1 chr3 72749277 72861914 - ZNF500 chr16 4748239 4767624 - UBN1 chr16 4846665 4882401 + CALR chr19 12938578 12944489 + ICE2 chr15 60419609 60479160 - L1TD1 chr1 62194849 62212328 + TRIP12 chr2 229763838 229923239 - ZNF43 chr19 21804949 21852125 - GMPS chr3 155870650 155944020 + ABCC1 chr16 15949577 16143074 + RPL36A-HNRNPH2 chrX 101391011 101412297 + BAMBI chr10 28677510 28682932 + NACA2 chr17 61590421 61591219 - ZZEF1 chr17 4004445 4143030 - PPP1R12C chr19 55090914 55117637 - TLE3 chr15 70047790 70098176 - NCL chr2 231453531 231483641 - DCAF10 chr9 37800554 37867666 + MIXL1 chr1 226223618 226227054 + NAT10 chr11 34105617 34147670 + ALDOA chr16 30064164 30070457 + MON1B chr16 77190835 77202398 + TIMMDC1 chr3 119498547 119525090 + SH3PXD2A chr10 103594027 103855543 - ATF6B chr6 32115264 32128253 - CDK7 chr5 69234795 69277430 + RIPOR1 chr16 67518418 67546788 + NPC2 chr14 74476192 74494177 - CACNG6 chr19 53992288 54012669 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL355075.4 chr14 20343048 20343685 - LINC01248 chr2 5602505 5691488 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.