exRNAdisease

Entry Detail

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General Information

Database ID: exR0359548 RNA Name: AKAP9 RNA Type: mRNA Chromosome: chr7 Starnd: + Coordinate: Start Site(bp): 91940867 End Site(bp): 92110673 External Links: ENSG00000127914

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CCDC85C chr14 99500190 99604207 - SFRP1 chr8 41261962 41309473 - YWHAE chr17 1344275 1400222 - RBBP6 chr16 24537693 24572863 + SMARCC1 chr3 47585269 47782106 - PABPC1 chr8 100685816 100722809 - C2orf68 chr2 85605254 85612066 - FKBP4 chr12 2794970 2805423 + KDM5C chrX 53191321 53225422 - RHOA chr3 49359145 49412998 - U2AF1 chr21 43092956 43107587 - PIGQ chr16 566995 584136 + ALDOA chr16 30064164 30070457 + UBE2C chr20 45812576 45816957 + ZDHHC9 chrX 129803288 129843909 - TPT1 chr13 45333471 45341370 - CNPY3 chr6 42929192 42939294 + OXA1L chr14 22766522 22773042 + HMGA2 chr12 65824131 65966295 + UQCC2 chr6 33694293 33711727 - KMT2B chr19 35717973 35738878 + MRPL42 chr12 93467488 93516214 + RPS13 chr11 17074388 17077715 - SALL3 chr18 78980275 79002677 + AUP1 chr2 74526645 74529760 - ILF2 chr1 153661788 153671028 - CBX3 chr7 26201162 26213607 + TUBB chr6 30720352 30725426 + C12orf10 chr12 53299695 53307177 + MEX3D chr19 1554669 1568058 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand Z98883.1 chr16 568837 576257 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.