exRNAdisease

Entry Detail

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General Information

Database ID: exR0359549 RNA Name: AKIP1 RNA Type: mRNA Chromosome: chr11 Starnd: + Coordinate: Start Site(bp): 8911139 End Site(bp): 8920084 External Links: ENSG00000166452

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand CBX3 chr7 26201162 26213607 + FSCN1 chr7 5592816 5606655 + MDM2 chr12 68808177 68850686 + OAS3 chr12 112938444 112973251 + COPS7B chr2 231781671 231809254 + EIF1 chr17 41688885 41692668 + MRPL49 chr11 65122183 65127371 + SLC2A1 chr1 42925375 42958868 - B2M chr15 44711487 44718877 + NIPSNAP1 chr22 29554808 29581327 - AK4 chr1 65147549 65232145 + SLC25A6 chrX 1386152 1392113 - DHX15 chr4 24517441 24584554 - VLDLR chr9 2621787 2660056 + AL136454.1 chr1 192716132 192716653 + TXNL1 chr18 56597209 56651600 - CHKB-CPT1B chr22 50568869 50582965 - KRT7 chr12 52232520 52252186 + TAGLN2 chr1 159918107 159925732 - CHMP1A chr16 89644435 89657721 - FP565260.6 chr21 5155499 5165472 - FP565260.2 chr21 5116343 5133805 - OST4 chr2 27070472 27071654 - TSTD2 chr9 97600080 97633368 - PSMA5 chr1 109399042 109426448 - PPP2R1A chr19 52190048 52229518 + IL21R chr16 27402174 27452042 + THOC5 chr22 29505879 29555216 - GPSM3 chr6 32190766 32195523 - MAT2A chr2 85539168 85545281 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-4426 chr1 192716333 192716349 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand DANCR chr4 52712404 52720351 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.