exRNAdisease

Entry Detail

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General Information

Database ID: exR0359564 RNA Name: AKT1 RNA Type: mRNA Chromosome: chr14 Starnd: - Coordinate: Start Site(bp): 104769349 End Site(bp): 104795751 External Links: ENSG00000142208

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand PPM1B chr2 44167969 44244384 + KEAP1 chr19 10486125 10503558 - AC010422.3 chr19 12643831 12648397 - FKBP1A chr20 1368978 1393172 - ACACA chr17 37084992 37406836 - TUFM chr16 28842411 28846348 - SCFD2 chr4 52872982 53366066 - P4HB chr17 81843161 81860624 - CRELD1 chr3 9933816 9945406 + SIGIRR chr11 405716 417455 - KDM4B chr19 4969113 5153598 + TMEM106C chr12 47963569 47968878 + FAT1 chr4 186587794 186726722 - POU5F1 chr6 31164337 31180731 - INTS5 chr11 62646848 62653302 - ZC3H14 chr14 88562970 88627596 + TPI1 chr12 6867119 6870948 + CA14 chr1 150257251 150265078 + KDELR3 chr22 38468078 38483447 + DLG3 chrX 70444835 70505490 + FBRSL1 chr12 132489551 132585188 + MOV10 chr1 112673141 112700746 + ENAH chr1 225486835 225653142 - EEF1A1 chr6 73515750 73523797 - RARRES2 chr7 150338317 150341662 - MVD chr16 88651935 88663161 - TUBB chr6 30720352 30725426 + HNRNPL chr19 38836388 38852347 - HPCAL1 chr2 10302889 10427617 + PPA1 chr10 70202835 70233911 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004816.1 chr14 70809841 70815994 + AC012313.3 chr19 58346854 58362751 - AC067930.1 chr8 143579636 143580670 + GSEC chr11 126340889 126355587 - MIR663AHG chr20 26167817 26251546 - PDCD4-AS1 chr10 110869868 110872233 - XIST chrX 73820649 73852723 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.