exRNAdisease

Entry Detail

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General Information

Database ID: exR0359567 RNA Name: AKT3 RNA Type: mRNA Chromosome: chr1 Starnd: - Coordinate: Start Site(bp): 243488233 End Site(bp): 243851079 External Links: ENSG00000117020

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand NUP107 chr12 68686951 68745809 + FUCA1 chr1 23845077 23868294 - HNRNPK chr9 83968083 83980616 - RPL37 chr5 40825262 40835222 - EDEM2 chr20 35115364 35147336 - PRDX6 chr1 173477330 173488815 + HLA-C chr6 31268749 31272130 - MAP3K7 chr6 90513573 90587072 - SRSF4 chr1 29147743 29181900 - IPMK chr10 58191517 58267894 - POLR2F chr22 37952607 38041915 + IST1 chr16 71845996 71931199 + ID1 chr20 31605283 31606515 + L1TD1 chr1 62194849 62212328 + SF3B1 chr2 197388515 197435091 - SDCCAG8 chr1 243256034 243500091 + POLR1D chr13 27620742 27744237 + ESRP1 chr8 94641074 94707466 + LAPTM4B chr8 97775057 97853013 + AKT2 chr19 40230317 40285536 - STK4 chr20 44966479 45080021 + HNRNPL chr19 38836388 38852347 - PAPOLA chr14 96501433 96567111 + PKN1 chr19 14433053 14471867 + IL6R chr1 154405193 154469450 + TIMM10B chr11 6481501 6484681 + RPL13A chr19 49487554 49492308 + RTN4R chr22 20241415 20283246 - ANKFY1 chr17 4163821 4263995 - SGSH chr17 80206716 80220923 - miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand RMRP chr9 35657751 35658018 - SNHG1 chr11 62851984 62855953 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.