exRNAdisease

Entry Detail

---



General Information

Database ID: exR0359571 RNA Name: ALCAM RNA Type: mRNA Chromosome: chr3 Starnd: + Coordinate: Start Site(bp): 105366909 End Site(bp): 105576900 External Links: ENSG00000170017

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ZNF525 chr19 53365693 53392217 + RPL34 chr4 108620566 108630412 + EIF5A chr17 7306999 7312463 + OAZ1 chr19 2269509 2273490 + MT-CO1 chrMT 5904 7445 + SLC10A7 chr4 146253975 146521964 - FBXO45 chr3 196568611 196589059 + ZNF664 chr12 123971845 124015439 + EIF3F chr11 7970251 8001862 + NUDT5 chr10 12165330 12196144 - NOP56 chr20 2652593 2658393 + CMTM6 chr3 32481312 32502852 - POLR2E chr19 1086574 1095380 - FRAS1 chr4 78057323 78544269 + VAMP3 chr1 7771296 7781432 + ATP5MG chr11 118401606 118431496 + FKBP8 chr19 18531751 18544077 - TMEM107 chr17 8172457 8176399 - ADK chr10 74151202 74709963 + CYTIP chr2 157414619 157488961 - CDH3 chr16 68636189 68722616 + KBTBD2 chr7 32868172 32894131 - EIF3E chr8 108201216 108435333 - RPLP0 chr12 120196699 120201235 - PRRC2B chr9 131373636 131500197 + RBM5 chr3 50088919 50119021 + PPME1 chr11 74171267 74254703 + BTG1 chr12 92140278 92145846 - IGFBP2 chr2 216632828 216664436 + SPRED1 chr15 38252836 38357249 + miRNA targets: NA circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC024940.6 chr12 31280422 31280895 - AD000090.1 chr19 35557956 35581954 + LINC00992 chr5 117415509 117546298 + MALAT1 chr11 65497688 65506516 + PRNCR1 chr8 127079874 127092600 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.