exRNAdisease

Entry Detail

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General Information

Database ID: exR0359581 RNA Name: ALDH3A2 RNA Type: mRNA Chromosome: chr17 Starnd: + Coordinate: Start Site(bp): 19648136 End Site(bp): 19685760 External Links: ENSG00000072210

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand BLM chr15 90717346 90816166 + C4orf3 chr4 119296419 119304445 - KMT2D chr12 49018975 49059774 - EIF5A chr17 7306999 7312463 + PRSS16 chr6 27247701 27256624 + NPM1 chr5 171387116 171411137 + ERGIC3 chr20 35542021 35557634 + BST2 chr19 17402939 17405630 - IQGAP1 chr15 90388242 90502239 + HMGCR chr5 75336329 75362101 + MTRNR2L3 chr20 57358447 57359498 - PEA15 chr1 160205337 160215376 + BOD1 chr5 173607145 173616659 - TUBA1A chr12 49184795 49189080 - SPCS1 chr3 52704955 52711148 + DGLUCY chr14 91060333 91225632 + GID8 chr20 62938147 62948475 + ENSA chr1 150600851 150629612 - RSL24D1 chr15 55180806 55197049 - ARPC2 chr2 218217141 218254356 + ZNF431 chr19 21142024 21196053 + MFGE8 chr15 88898683 88913381 - MIF chr22 23894383 23895227 + MPC2 chr1 167916675 167937072 - TNFRSF14 chr1 2555639 2565382 + SOX8 chr16 981770 986979 + PDZD8 chr10 117277274 117375440 - BCR chr22 23179704 23318037 + AK2 chr1 33007940 33080996 - AMACR chr5 33986165 34008104 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-19b-3p chr13 91351245 91351267 + hsa-miR-27a-3p chr19 13836447 13836467 - hsa-miR-27b-3p chr9 95085505 95085525 + hsa-miR-301a-3p chr17 59151149 59151171 - hsa-miR-431-5p chr14 100881026 100881046 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand SCARNA9 chr11 93721513 93721865 + SNHG16 chr17 76557764 76565348 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.