exRNAdisease

Entry Detail

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General Information

Database ID: exR0359583 RNA Name: ALDH5A1 RNA Type: mRNA Chromosome: chr6 Starnd: + Coordinate: Start Site(bp): 24494867 End Site(bp): 24537207 External Links: ENSG00000112294

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand YWHAB chr20 44885702 44908532 + B2M chr15 44711487 44718877 + SLC25A6 chrX 1386152 1392113 - MAP7D1 chr1 36155579 36180849 + TTC19 chr17 15999784 16045015 + EIF3E chr8 108201216 108435333 - FBRSL1 chr12 132489551 132585188 + IRF2BP2 chr1 234604269 234609525 - USP53 chr4 119212587 119295517 + SH3GLB2 chr9 129007036 129028303 - MYO10 chr5 16661907 16936288 - TET3 chr2 73986404 74108176 + STK11IP chr2 219597857 219616451 + NFKB2 chr10 102394110 102402524 + MAPK12 chr22 50245450 50261716 - ERRFI1 chr1 8004404 8026309 - PNMA2 chr8 26504701 26514092 - HUWE1 chrX 53532096 53686728 - GTF2F1 chr19 6379572 6393981 - RPS6 chr9 19375715 19380236 - RPL37A chr2 216498825 216579180 + SLX1A chr16 30193887 30197561 + BAX chr19 48954815 48961798 + IGF2 chr11 2129112 2141238 - KLC4 chr6 43040777 43075095 + ZNF33A chr10 38010650 38065088 + ZNF12 chr7 6688433 6706947 - LASP1 chr17 38869859 38921770 + RPL13 chr16 89560657 89566828 + FAM104B chrX 55143102 55161310 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-129-5p chr7 128207876 128207896 + hsa-miR-330-5p chr19 45639049 45639070 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC022201.2 chr2 70301451 70302072 + AL645941.1 chr6 32970232 32970886 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.