exRNAdisease

Entry Detail

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General Information

Database ID: exR0360744 RNA Name: CALM3 RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 46601074 End Site(bp): 46610782 External Links: ENSG00000160014

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ALDH6A1 chr14 74056847 74084492 - ADAMTSL4 chr1 150549369 150560937 + ABHD12 chr20 25294742 25390835 - AGPAT1 chr6 32168212 32178096 - ACOT13 chr6 24667035 24705065 + AHCY chr20 34280268 34311802 - AGO1 chr1 35869808 35930532 + ALDH7A1 chr5 126531200 126595362 - AAMDC chr11 77821109 77918432 + ADAR chr1 154582057 154628013 - AHSA1 chr14 77457870 77469472 + AC006486.1 chr19 42234583 42255132 - AC010422.3 chr19 12643831 12648397 - ABI2 chr2 203328239 203447728 + AL121594.1 chr14 35122549 35317474 + ALDH18A1 chr10 95605941 95656711 - AC099811.2 chr17 42119674 42154916 - ABCC5 chr3 183919934 184017939 - ACTG1 chr17 81509971 81523847 - AIF1L chr9 131096476 131123152 + AC245033.1 chr15 82536788 82573194 - ACACA chr17 37084992 37406836 - ADAM19 chr5 157395534 157575775 - ABTB1 chr3 127672935 127680926 + ACTB chr7 5527148 5563784 - ACIN1 chr14 23058564 23095614 - AGAP1 chr2 235494043 236131800 + AC009133.6 chr16 29812261 29820092 + AARSD1 chr17 42950526 42964498 - AC090360.1 chr18 80034346 80097088 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-34a-5p chr1 9151735 9151756 - hsa-miR-205-5p chr1 209432166 209432187 + hsa-miR-449a chr5 55170586 55170607 - hsa-miR-449b-5p chr5 55170706 55170727 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC016876.2 chr17 7581964 7584086 - AC026362.1 chr12 122975320 122982907 + AC079781.5 chr7 97851688 97972985 - AC093503.2 chr19 46609277 46610779 - AC125807.2 chr12 3041437 3044950 + AD000090.1 chr19 35557956 35581954 + AL022311.1 chr22 37876148 37895563 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.