exRNAdisease

Entry Detail

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General Information

Database ID: exR0360749 RNA Name: CALR RNA Type: mRNA Chromosome: chr19 Starnd: + Coordinate: Start Site(bp): 12938578 End Site(bp): 12944489 External Links: ENSG00000179218

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AARSD1 chr17 42950526 42964498 - ABCF2 chr7 151207837 151227166 - ABHD14B chr3 51968510 51983409 - ABI1 chr10 26746593 26861087 - ABRACL chr6 139028745 139043302 + AC005943.1 chr19 1578339 1605445 - AC007192.1 chr19 18153158 18178117 + AC069503.2 chr12 121888809 121921470 + AC138811.2 chr16 18788063 18801519 - ACAD8 chr11 134253495 134265855 + ACTB chr7 5527148 5563784 - ACTG1 chr17 81509971 81523847 - ACTR1A chr10 102461881 102502712 - ACTR3 chr2 113890063 113962596 + ADCYAP1R1 chr7 31052461 31111479 + ADNP2 chr18 80109262 80147523 + AHCY chr20 34280268 34311802 - AK3 chr9 4709556 4742043 - AKAP8 chr19 15353385 15379798 - AKT2 chr19 40230317 40285536 - ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-663a chr20 26208243 26208264 - hsa-miR-423-5p chr17 30117095 30117117 + hsa-miR-541-3p chr14 101064548 101064569 + hsa-miR-6515-5p chr19 12940486 12940506 + hsa-miR-6515-3p chr19 12940521 12940540 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC012236.1 chr15 31221999 31230838 - AC026362.1 chr12 122975320 122982907 + AC064802.1 chr8 114282067 114295839 + AC132217.1 chr11 2129121 2129964 - AD000090.1 chr19 35557956 35581954 + AL021707.1 chr22 38739003 38749041 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.