exRNAdisease

Entry Detail

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General Information

Database ID: exR0360842 RNA Name: CBS RNA Type: mRNA Chromosome: chr21 Starnd: - Coordinate: Start Site(bp): 43053191 End Site(bp): 43076943 External Links: ENSG00000160200

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABHD16A chr6 31686955 31703356 - ABHD4 chr14 22598237 22613215 + ABHD5 chr3 43690108 43734371 + AC010323.1 chr19 8308283 8321379 - AC010422.5 chr19 12664828 12669397 - AC135050.2 chr16 31083439 31094956 - AC138811.2 chr16 18788063 18801519 - AC139530.2 chr17 81703371 81720539 + AC245033.1 chr15 82536788 82573194 - ACKR3 chr2 236567787 236582354 + ACTA1 chr1 229431245 229434098 - ACTG1 chr17 81509971 81523847 - ACTR1A chr10 102461881 102502712 - ACTR3 chr2 113890063 113962596 + ADRM1 chr20 62302093 62308862 + AGBL5 chr2 27042364 27070622 + AKAP1 chr17 57085092 57121346 + AKR1A1 chr1 45550543 45570049 + AL132780.3 chr14 22946270 22982258 - AL162417.1 chr9 133098121 133163914 - ALDH9A1 chr1 165662216 165698863 - ALDOA chr16 30064164 30070457 + miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-6853-3p chr9 35732974 35732995 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC004951.4 chr7 43951910 44019151 - AC026461.1 chr16 56682470 56687807 + AC069281.2 chr7 100572232 100578700 - AC074044.1 chr3 112990447 112991153 - AC245033.4 chr15 82533175 82540008 - AD000090.1 chr19 35557956 35581954 + AL049796.1 chr1 93847174 93848939 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.