exRNAdisease

Entry Detail

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General Information

Database ID: exR0362126 RNA Name: DDX5 RNA Type: mRNA Chromosome: chr17 Starnd: - Coordinate: Start Site(bp): 64498254 End Site(bp): 64508199 External Links: ENSG00000108654

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand ABCB4 chr7 87401697 87480435 - ABCB7 chrX 75051048 75156732 - ABCF2 chr7 151207837 151227166 - AC005258.1 chr19 2269525 2341172 + AC010132.3 chr7 42909273 42932174 - AC010422.3 chr19 12643831 12648397 - AC024592.3 chr19 5866171 5903787 - AC048338.1 chr12 122207779 122266423 - AC093525.2 chr16 2496032 2520218 + AC120057.2 chr17 7240427 7244635 - AC245033.1 chr15 82536788 82573194 - ACOT13 chr6 24667035 24705065 + ACSS2 chr20 34872146 34927962 + ADA2 chr22 17178790 17258235 - ADIPOR2 chr12 1688574 1788674 + AFDN chr6 167826922 167972023 + AGFG2 chr7 100539203 100568220 + AGO1 chr1 35869808 35930532 + AGO4 chr1 35808016 35857890 + AGPAT5 chr8 6708642 6761503 + AHDC1 chr1 27534035 27604431 - AKAP12 chr6 151239967 151358559 + AKIRIN1 chr1 38991276 39006059 + AL133352.1 chr10 100505628 100529881 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-18a-5p chr13 91350756 91350778 + hsa-miR-33a-5p chr22 41900949 41900969 + hsa-miR-141-3p chr12 6964155 6964176 + hsa-miR-206 chr6 52144401 52144422 + hsa-miR-302b-3p chr4 112648489 112648511 - hsa-miR-33b-5p chr17 17813897 17813916 - hsa-miR-3064-5p chr17 64500817 64500837 - hsa-miR-3064-3p chr17 64500775 64500797 - hsa-miR-5047 chr17 64501230 64501250 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC020663.1 chr16 4730115 4752565 - AC023509.1 chr12 53441741 53467528 + AC079781.5 chr7 97851688 97972985 - Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.