exRNAdisease

Entry Detail

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General Information

Database ID: exR0372027 RNA Name: VCP RNA Type: mRNA Chromosome: chr9 Starnd: - Coordinate: Start Site(bp): 35056064 End Site(bp): 35072627 External Links: ENSG00000165280

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: Gene Symbol Chromosome Start Site(bp) End Site(bp) Strand AACS chr12 125065434 125143333 + ABHD12 chr20 25294742 25390835 - AC005258.1 chr19 2269525 2341172 + AC010422.3 chr19 12643831 12648397 - AC073896.1 chr12 56285916 56316059 - AC091167.2 chr15 90249556 90272208 + AC093525.2 chr16 2496032 2520218 + ACADS chr12 120725774 120740008 + ACBD6 chr1 180269653 180502954 - ACTG1 chr17 81509971 81523847 - ADAR chr1 154582057 154628013 - ADH5 chr4 99070978 99088801 - AL121753.1 chr20 35267885 35280043 - ALDH2 chr12 111766887 111817532 + ALDH7A1 chr5 126531200 126595362 - miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-331-3p chr12 95308480 95308500 + hsa-miR-339-5p chr7 1022990 1023012 - hsa-miR-520a-5p chr19 53690895 53690915 + hsa-miR-450b-5p chrX 134540231 134540252 - circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AC002310.1 chr16 30534752 30537149 + AC112484.1 chr3 128860620 128871540 - AC132217.1 chr11 2129121 2129964 - AC245014.3 chr1 145281116 145281462 + AD000090.1 chr19 35557956 35581954 + AL031282.2 chr1 1702736 1737688 - AL121992.1 chr1 15586136 15603626 - AL135925.1 chr10 79825902 79827602 + AL136985.3 chr1 58715609 58771295 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 17787 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.