exRNAdisease

Entry Detail

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General Information

Database ID: exR0373243 RNA Name: NUTM2A-AS1 RNA Type: lncRNA Chromosome: chr10 Starnd: - Coordinate: Start Site(bp): 87201647 End Site(bp): 87342612 External Links: ENSG00000223482

Disease Information

Disease Name: Amyotrophic Lateral Sclerosis Disease Category: Nervous System Diseases MeSH ID: D000690 Type: Diseases Category/Nervous System Diseases Alias: Sclerosis, Amyotrophic Lateral//Gehrig's Disease//Gehrig Disease//Gehrigs Disease//Charcot Disease//Motor Neuron Disease, Amyotrophic Lateral Sclerosis//Lou Gehrig's Disease//Lou-Gehrigs Disease//Disease, Lou-Gehrigs//ALS - Amyotrophic Lateral Sclerosis//ALS Amyotrophic Lateral Sclerosis//Lou Gehrig Disease//Amyotrophic Lateral Sclerosis, Guam Form//Amyotrophic Lateral Sclerosis-Parkinsonism-Dementia Complex 1//Amyotrophic Lateral Sclerosis Parkinsonism Dementia Complex 1//Guam Form of Amyotrophic Lateral Sclerosis//Guam Disease//Disease, Guam//Amyotrophic Lateral Sclerosis, Parkinsonism-Dementia Complex of Guam//Amyotrophic Lateral Sclerosis, Parkinsonism Dementia Complex of Guam//Amyotrophic Lateral Sclerosis With Dementia//Dementia With Amyotrophic Lateral Sclerosis

Expression Detail

GEO ID: GSE121519 Description: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid. Experimental Design: Disease vs Control Case Disease Type: Amyotrophic Lateral Sclerosis Case Disease SubType: NA Case Sample: Amyotrophic Lateral Sclerosis Control Sample: Healthy Number of Case: 4 Number of Control: 4 Number of Samples: 8

Regulatory Relationship

mRNA targets: NA miRNA targets: miRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand hsa-miR-148b-3p chr12 54337278 54337299 + hsa-let-7a-5p chr9 94175962 94175983 + hsa-miR-4428 chr1 237471164 237471185 + hsa-miR-4458 chr5 8460933 8460951 + hsa-miR-107 chr10 89592756 89592778 - hsa-miR-129-5p chr7 128207876 128207896 + hsa-let-7c-5p chr21 16539838 16539859 + hsa-miR-15a-5p chr13 50049167 50049188 - hsa-miR-376a-3p chr14 101040825 101040845 + hsa-miR-424-5p chrX 134546680 134546701 - hsa-miR-16-5p chr3 160404754 160404775 + hsa-miR-103a-3p chr5 168560904 168560926 - hsa-miR-139-5p chr11 72615102 72615124 - hsa-let-7d-5p chr9 94178841 94178862 + hsa-let-7b-5p chr22 46113691 46113712 + hsa-miR-488-3p chr1 177029374 177029394 - hsa-miR-148a-3p chr7 25949922 25949943 - hsa-let-7e-5p chr19 51692793 51692814 + hsa-miR-942-5p chr1 117094655 117094676 + hsa-let-7i-5p chr12 62603691 62603712 + hsa-miR-4524a-5p chr17 69099606 69099627 - hsa-miR-6884-5p chr17 40026383 40026404 - hsa-miR-4524b-5p chr17 69099570 69099590 + hsa-miR-548o-3p chr20 38516607 38516628 + hsa-miR-4500 chr13 87618671 87618687 - hsa-miR-409-5p chr14 101065314 101065336 + hsa-miR-485-5p chr14 101055427 101055448 + hsa-miR-6838-5p chr7 44073407 44073428 - hsa-miR-195-5p chr17 7017667 7017687 - hsa-miR-15b-5p chr3 160404607 160404628 + circRNA targets: NA lncRNA targets: lncRNA Symbol Chromosome Start Site(bp) End Site(bp) Strand AL132656.3 chr10 79663192 79664786 + Display:

Experiment Detail

GEO ID: GSE121519 Sample Source: Cerebrospinal Fluid Source Fraction: Exosome Platform: GPL18573 Method: NGS Num of detected RNA Type: 2 Num of detected RNAs of this Type: 945 Sample treatment protocol: Up to 1 mL of human CSF was centrifuged at 2,000×g for 5 min and 10, 000×g for 20 min for supernatant. RNA Extract protocol: Supernatant from final centrifugation was applied to exoRNeasy spin column and RNA was extracted. RNA library preparation protocol: Whole cDNA amplification was performed with SMART-seq v4 Ultra Low Input RNA Kit. Illumina library was constructed with Nextera XT DNA Library Prep Kit.

Reference

PMID: 30630471 Title: Identification of biomarkers for amyotrophic lateral sclerosis by comprehensive analysis of exosomal mRNAs in human cerebrospinal fluid Author: Otake K, Kamiguchi H, Hirozane Y Journal: BMC Med Genomics. 2019 Jan 10;12(1):7. Description: The main purpose of this study is to established the methodology of comprehensive analysis of exosomal mRNAs in CSF by a highly sensitive next-generation sequencing.